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Copyright 2003
Center for Biotechnology and Genomic Medicine
Medical College of Georgia
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    Data Analysis
  • We are currently using primarily two clustering packages to analyze our  microarray data.
  • The first is Cluster by Michael Eisen.
         This package will let the investigator analyze multiple datasets simultaneously
         (e.g. a time course study), filter the dataset (e.g. use only data > 3 SD), adjust the
         dataset (e.g. normalize) and cluster the dataset. The clustering options in the program
         are hierarchical clustering, K-mean clustering, Self-Organizing Maps, and principle
         component analysis. Each of these methods have parameters useful to set a threshold of
         significance. The tab-delimited text file output of Cluster can be read by another
         companion program called Treeview. This program is an interactive graphical analysis
         package designed to view data generated by Cluster. The GUI interface presents the data
         in a tree format next to a color representation of the clustered array data and allows the
         investigator to quickly zoom in and identify the genes in specific clusters.
  • The second is Genecluster by Golub et al..
         This computer program will create self-organizing maps (SOM) using gene expression
         data. Additionally, the package will let one filter and normalize the data across multiple
         array experiments as well as visualize the clusters.
  • Alternative commercial packages are also being evaluated.
  •                
            Workflow
  • We are in the process of creating web based workflow schemes to manage the array workflow.
  • Visit again soon for more information!
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