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Data Analysis
We are currently using primarily two clustering packages to analyze our microarray data.
The first is Cluster by Michael Eisen.
This package will let the investigator analyze multiple datasets simultaneously
(e.g. a time course study), filter the dataset (e.g. use only data > 3 SD), adjust the
dataset (e.g. normalize) and cluster the dataset. The clustering options in the program
are hierarchical clustering, K-mean clustering, Self-Organizing Maps, and principle
component analysis. Each of these methods have parameters useful to set a threshold of
significance. The tab-delimited text file output of Cluster can be read by another
companion program called Treeview. This program is an interactive graphical analysis
package designed to view data generated by Cluster. The GUI interface presents the data
in a tree format next to a color representation of the clustered array data and allows the
investigator to quickly zoom in and identify the genes in specific clusters.
The second is Genecluster by Golub et al..
This computer program will create self-organizing maps (SOM) using gene expression
data. Additionally, the package will let one filter and normalize the data across multiple
array experiments as well as visualize the clusters.
Alternative commercial packages are also being evaluated. |
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Workflow
We are in the process of creating web based workflow schemes to manage the array workflow.
Visit again soon for more information! |
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What's new
You can check here to see what is new in the system. We will post items in this section for your convenience.
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Publications
We will list any interesting publications here.
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